CORESTA Congress, 13-17 October 2024, Edinburgh, Scotland – UK
M. Hollings1, E. Rothwell1, R. Bedford1, N. Haydare2, B. Ghiot2, L. Scott2, R. Mahendran2 and V. Hutter2;
1Labcorp, Harrogate, UK;
2ImmuONE Ltd, Stevenage, UK
Summary
ImmuLUNG cultures were exposed to whole smoke (WS) generated from 1R6F Kentucky Reference using a Vitrocell® VC10® smoking robot at varying airflows. The effects of WS on cytotoxicity, oxidative stress, epithelial barrier permeability, cytokine secretion, gene expression and individual cell phenotype via high content imaging analysis were assessed. The ImmuLUNG epithelial component exhibited high TEER values. Concentration-dependent viability and oxidative stress responses were observed, as indicated by 8-isoprostane/LDH release. Gene expression analysis revealed relevant macrophage RNA markers and upregulation of genes controlling macrophage production, differentiation, and function. Proteomic analysis showed increased expression of inflammatory signaling proteins and protein expression changes associated with oxidative stress control. High- content image analysis of ImmuLUNG macrophages revealed morphological changes, indicating assessment of macrophage endpoints are compatible with WS exposure.
