VITROCELL® Cloud Alpha MOVE

For fully automated and repeated row-by-row exposure of 12-, 24- and 96-well sized cell culture inserts.
Testing of up to 12 dose ranges in one experiment.

The VITROCELL^® Cloud Alpha MOVE presents another great leap forward in fully automated exposure of cell cultures. It is the ideal device to obtain a dose-response relationship in one experiment. The development is based on the well-known and frequently published VITROCELL^® Cloud formats. It’s functionality enables a fully automated process with an all-in-one control unit.

Developed with a focus on automation

The Cloud Alpha MOVE is capable to expose mammalian cell cultures in Corning^® HTS Transwell^® 96-Well Permeable Support Systems as well as 12-and 24-well sized inserts. The Cloud system is suitable for nebulization of solutions and suspensions. Fields of application are screening of inhaled drugs, toxicity testing of inhaled substances such as chemicals or nanoparticles and virus research.

Choice of three types of nebulizers

It comes with a choice of 3 types of vibrating mesh nebulizers having droplet MMAD ranges of 2.5–6.0 µm, 2.5–4.0 µm, 4.0–6.0 µm and in a special version with 9.0–12.0 µm. The device is particularly suitable for testing whenever small quantitities of testing materials are available.

All VITROCELL^® Cloud Alpha exposure devices have been specifically designed to enable direct exposure of mammalian cells or tissue at the Air/Liquid Interface in physiologically relevant conditions. Here the cell cultures are not covered with media as opposed to submerged conditions which cause an undesired interaction of the formerly airborne substances with the culture media.

This approach allows for more credible and authentic results than by submerged exposure due to a closer replication of the human physiology.

Exposure at the Air/Liquid Interface

Air/Liquid Cultivation
and Exposure in Exposure Module

A: Direct and controlled exposure of test atmosphere to cells
B: Cells on membrane
C: Media below cells

96-well: 12 Individual Doses

Sequential Exposure of Corning® HTS Transwell® 96-Well Permeable Support Systems in up to
12 steps @8 replicates.

24-well: 6 Individual Doses

Sequential Exposure of 24-well sized culture inserts in up to 6 steps @4 replicates.

12-well: 4 Individual Doses

Sequential Exposure of 12-well sized culture inserts in up to 4 steps@3 replicates.

For each of the exposures applies: After each nebulization the exposure chamber is automatically evacuated by vacuum pump to enable equal exposure conditions for the following nebulisation.

Discover more details in 3D-interaction

More about VITROCELL Cloud Alpha MOVE

Different choices of nebulizers

The system offers a selection of three types of vibrating mesh nebulizers, with droplet MMAD ranges of 4.0 – 6.0 µm, 4.0 – 6.0 µm, 2.5 – 6.0 µm, and 4.0–6.0 µm, 12 µm, 17 µm, and 20 µm.

With a recommended nebulization volume of 300 - 500 µL per exposure, the Cloud Alpha MOVE is especially well-suited for experiments where only small amounts of test material are available.

Dosimetry using Quartz Crystal Microbalance (QCM)

The Quartz Crystal Microbalance sensor is integrated in the Cloud Alpha MOVE exposure module. It is capable of measuring the deposited mass in nanogram/cm². Results are recorded in the VITROCELL^® Monitor software. Data is presented in graphs and stored in MS Excel^®.

Features

Fully automated row-by-row exposure of 12-, 24- and 96-well sized cell culture inserts
Aerosolisation defined by nebulization time or volume
Integrated controller for aerosol generator
Optional integrated microbalance controller
Programmable experiment recipes
Output rate database for nebulizers
Heating system
PowerVent function: evacuation of residual aerosols via vacuum pump
Designed for screening of inhaled drugs, toxicity testing of inhaled substances such as chemicals or nanoparticles and virus research

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